A Deoxyribonuclease Which Requires Nucleoside Triphosphate from Micrococcus lysodeikticus
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چکیده
منابع مشابه
A deoxyribonuclease which requires nucleoside triphosphate from Micrococcus lysodeikticus. II. Studies on the role of nucleoside triphosphate.
The ADP-ATP exchange reaction catalyzed by the highly purified Micrococcus lysodeikticus DNase has been lnvestigated in detail. The exchange activity is eluted together with the DNase activity from triethylaminoethylcellulose and the ratio between both activities is relatively constant in the peak fractions. The exchange reaction between [W4C]ADP and ATP has an absolute requirement for ATP and ...
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1. A method is described for the isolation of pure mesosomal membrane fractions from Micrococcus lysodeikticus. 2. Plasmolysis of cells, before wall digestion, was necessary for effective mesosome release. 3. The effect of mild shearing forces, temperature and time upon the release of mesosomal membrane from protoplasts was investigated. 4. The optimum yield of mesosomal membranes from stable p...
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Sangivamycin is incorporated into RNA in the reaction catalyzed by the RNA polymerase from Micrococcus lysodeikticus with the use of calf thymus DNA or the copolymer of deoxyadenosine and deoxythymidine (poly d(A-T)). The incorporation of sangivamycin 5’4riphosphate into RNA is dependent on primer, time, protein concentration, and Mn++ or Mg+f. When calf thymus DNA is primer, GTP, CTP, and UTP ...
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Field, A. K. (Cornell University, Ithaca, N.Y.) and H. B. Naylor. Induction of lysogenic Micrococcus lysodeikticus. J. Bacteriol. 84:1129-1133. 1962.-Between 50 and 60% of log-phase cells of Micrococcus lysodeikticus strain ML 53-40, lysogenic for N5 bacteriophage, were induced by an optimal dose of ultraviolet light. Induction of dilute cell suspensions caused a subsequent 200- to 800-fold inc...
متن کاملA long chain terpenyl pyrophosphate synthetase from Micrococcus lysodeikticus.
A new terpene pyrophosphate synthetase (Enzyme II) distinct from the previously reported geranylgeranyl pyrophosphate synthetase (Enzyme I) has been isolated from extracts of Micrococcus lysodeikticus and partially purified. Enzyme II catalyzes the elongation of terpenoid ally1 pyrophosphates by isopentenyl pyrophosphate to long chain products. The order of effectiveness of various ally1 pyroph...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63330-7